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BioSampler

While there is no standardised method for bioaerosol collection at this time, many organisations, including SKC, are researching improved measurement techniques. The patented BioSampler® is a result of SKC's work with leading scientists at the University of Cincinnati.

BioSampler

225-9593/-9595

  • Allows use of non-evaporating collection liquids
  • Patented swirling liquid collection method minimises re-aerosolisation and particle bounce
  • Preserves micro-organism integrity and viability
  • Reusable — can be autoclaved
  • Maintains constant sampling efficiency up to 8 hours
  • Collected bioaerosols can be analysed by a variety of methods
  • Complete glass construction allows easy cleaning, sterilising, autoclaving and reuse
  • Collection liquid easily transferred to agar plate for culturing

† U.S Patent No. 5,902,385 (device)
‡ U.S Patent No. 5,904,752 (method)

BioSampler Operation

The BioSampler® must be operated with a high volume sonic flow pump, such as the BioLite/BioLite+ Pump, that can maintain 15 inches mercury or 0.5 of an atmosphere of downstream pressure in the system. The BioSampler’s three tangential nozzles act as critical (sonic) orifices, each permitting 4.2 L/min of ambient air to pass through for a total flow rate of approximately 12.5 L/min. Collection liquid with a viscosity much higher than water, such as ViaTrap (special mineral oil), can be used with the BioSampler to provide constant collection efficiency over an 8 hour sampling period.

Applications
  • Indoor air quality investigations
  • Infection control in hospitals and veterinary clinics
  • Quantification of microorganisms in agricultural dust
  • Biological research
  • Infectious disease investigations in public buildings
  • Safety concerns in the food handling industry
  • Workplace exposures in industries such as pulp and paper mills or wastewater treatment plants
Specifications
Sample Time    Up to 8 hours
Sample Rate    Sonic flow through BioSampler nozzles (12.5 L/min)
Sample Pump    BioLite/BioLite+ Pump
Sample Media    Water or more viscous liquids such as ViaTrap mineral oil
Tubing    1/4 inch ID and 3/8 inch ID
Analysis Options

BioSampler samples provide many analysis options:

  • Growth Culture Analysis quantifies and characterises airborne cultural bacteria and fungi
  • Microscopic Analysis enumerates and provides limited identification of total airborne bacteria and fungi
  • Biochemical Assay quantifies biological compounds based on reaction to a chemical
  • Immunoassay quantifies airborne allergens based on antibodies binding to a specific target antigen
  • Polymerase Chain Reaction (PCR) identifies bioaerosols by screening for a specific genus or species
BioSamplers
Description Part Number
20 ml BioSampler (Single), 3-piece glass, includes:

inlet section, outlet section, and collection vessel (does not include ground joint cap). Inlet and outlet sections are a matched set

225-9595
20 ml BioSampler (Set of 4), 3-piece glass, includes:

inlet section, outlet section, and collection vessel (does not include ground joint cap). Inlet and outlet sections are a matched set

225-9595K4
5 ml BioSampler, 3-piece glass, includes:

inlet section, outlet section, and collection vessel (does not include ground joint cap). Inlet and outlet sections are a matched set

225-9593
BioSampler Kits
Description Part Number
BioSampler Mini Kit includes:

BioSampler, two 20 ml collection vessels (bottoms) with caps, 1 BioSampler case with mounting rod, and 1 ViaTrap (120 ml).

Viatrap mineral oil may not be suitable for PCR analysis

225-9597
BioLite+ BioSampler Kit 230V includes:

BioLite+ 230V pump, 1 BioSampler with collection vessel, one additional 20 ml collection vessel with cap, 1 mounting bracket, tubing/adapters and rotameter

228-9620KB
BioLite+ BioSampler Deluxe Kit 230V includes:

BioLite+ 230V pump, 1 BioSampler, two 20 ml collection vessels with caps, 1 case with mounting rod, 1 ViaTrap* (120 ml), tubing/adapters and rotameter

228-9620KBD
BioLite+ BioSampler Kit 120V includes:

BioLite+ 120V pump, 1 BioSampler with collection vessel, one additional 20 ml collection vessel with cap, 1 mounting bracket, tubing/adapters and rotameter

228-9615KB
BioLite+ BioSampler Deluxe Kit 120V includes:

BioLite+ 120V pump, 1 BioSampler, two 20 ml collection vessels with caps, 1 case with mounting rod, 1 ViaTrap* (120 ml), tubing/adapters and rotameter

228-9615KBD

* May not be suitable for PCR analysis

BioSampler Accessories
Description Part Number  
20 ml BioSampler Collection Vessel (bottom) and ground joint cap, for transporting samples 225-9596  225-9596 20 ml BioSampler Collection Vessel (bottom) and ground joint cap, for transporting samples 
5 ml BioSampler Collection Vessel (bottom) and ground joint cap, for transporting samples 225-9596A  225-9596A 5 ml BioSampler Collection Vessel (bottom) and ground joint cap, for transporting samples 
120 ml ViaTrap Collection Media, special mineral oil for bioaerosol sampling.

Viatrap mineral oil may not be suitable for PCR analysis

225-9598A  225-9598A 120 ml ViaTrap Collection Media, special mineral oil for bioaerosol sampling. 
500 ml ViaTrap Collection Media, special mineral oil for bioaerosol sampling.

Viatrap mineral oil may not be suitable for PCR analysis

225-9598  225-9598 500 ml ViaTrap Collection Media, special mineral oil for bioaerosol sampling. 
950 ml ViaTrap Collection Media, special mineral oil for bioaerosol sampling.

Viatrap mineral oil may not be suitable for PCR analysis

225-9599  225-9599 950 ml ViaTrap Collection Media, special mineral oil for bioaerosol sampling. 
BioSampler Case with Mounting Rod, includes foam insert. Case serves as a base and rod secures BioSampler during sampling 225-9606  225-9606 BioSampler Case with Mounting Rod, includes foam insert. 
Glass Trap for area sampling to protect pump 225-22  225-22 Glass Trap for area sampling to protect pump and can be used with or without trap sorbent 
Trap Sorbent 200 g, silica gel/activated charcoal mix to remove vapours 225-22-02  225-22-02 Trap Sorbent 200 g, silica gel/activated charcoal mix to remove vapours 
BioSampler Mounting Bracket for BioLite+/BioLite Pump 228-9611  228-9611 BioSampler Mounting Bracket for BioLite+/BioLite Pump 
20 ml BioSampler Collection Vessel (bottom) and ground joint cap, for transporting samples

225-9596

5 ml BioSampler Collection Vessel (bottom) and ground joint cap, for transporting samples

225-9596A

120 ml ViaTrap Collection Media, special mineral oil for bioaerosol sampling

225-9598

500 ml ViaTrap Collection Media, special mineral oil for bioaerosol sampling

225-9598

950 ml ViaTrap Collection Media, special mineral oil for bioaerosol sampling

225-9599

BioSampler Case with Mounting Rod, includes foam insert

225-9606

Glass Trap for area sampling to protect pump

225-22

Trap Sorbent 200 g, silica gel/activated charcoal mix to remove vapours

225-22-02

BioSampler Mounting Bracket for BioLite+/BioLite Pump

228-9611

Documentation
BioSampler Instructions   BioLite+ and Biosampler Brochure

BioSampler
Instructions

 

BioSampler & BioLite+
Brochure